In these cases, when absorption is high and the dilution of the samples is difficult, “buckets of short path length” are applied for use. Buckets with a short pad length with optical pad lengths of 1 mm, 2 mm, 3 mm and 5 mm can be easily purchased. The results obtained by analyzing toluene with a 1mm quartz bucket and a 10mm quartz bucket indicate that there is much less absorption saturation with the 1mm bucket than with the 10mm cell. This baking material is ideal for use in the visible spectral range and has a decent transmission range of 340 to 2,500 nm. Most applications will fall into the range and many experiments do not need the additional UV range (190 – 340 nm) available with quartz materials.
Uv vis cuvette for spectrophotometry is transparent for the wavelength of light in the UV fish ranges for measurements. As a result, the bucket itself does not cause problems during the examination. The solution should be placed in the light path of the UV/fish spectrophotometer. A micropipette is used to inject the sample directly into the bucket and the bucket is ready for use. A well-known application of the short-path length bucket is infrared range spectrophotometry measurement.
The Type 1FLUV10 cell weighs about $125, which is well below the market value of this bucket. Unlike our spectrophotometer buckets, there is no U-shaped alternative in our fluorescence line. An ideal baking material would be transparent to the target light beam and would not interact with the samples used in the measurement. Buckets are usually made of various transparent materials, such as optical glass, quartz, sapphire or optical plastic. Each material has unique translucent properties and it is important to know these characteristic optical properties before selecting the baking material. The concentration of a sample will also affect the choice of container, as each instrument has an upper limit of detection.
If wavelengths are used in the UV range, below about 300 nm, buckets of quartz glass or a special type of plastic should be used, which provide sufficient transparency in this range. The polystyrene trays are designed for testing over the entire visible spectral range from 340nm to 750nm. A uv vis cuvettes standard light path of 1 cm and recessed windows provide light transmission. You can reduce your test volumes to save on expensive reagents. They require only a filling volume of 1.2 ml in the size of 1.5 ml. Although plastic buckets can only be reused a few times, they are cost-effective.
The selection of different types of buckets is more than expected, even those buckets used for absorption measurements in the field of UV-Vis spectroscopy. Light paths smaller than 1 mm are available, but are for more specialized buckets, such as detachable cells or HPLC flow cells. IR quartz cuvettes are suitable for wavelengths from 250nm to 3,500nm. RNA, single-stranded DNA and oligonucleotides, a path length long enough to allow data measurements to fall within the linear measurement range of the instrument is recommended.